Investigation of the Change of pH Affecting Enzyme Activity

Introduction:Research Question:The purpose of this experiment is to look into a factor simulateing enzyme activity. Hypothesis:A change in the pH of hydrogen peroxide will have an tint on enzyme activity. Explanation of Hypothesis:Enzymes are catalytic globular proteins made by living things that regulate the movement of molecules with metabolic pathways, as well as speed up chemical responses without being changed. They speed up metabolic reactions by bring down energy barriers. Catalyst is a chemical factor that changes the evaluate of a reaction without being consumed by the reaction (IB biological science notes). Enzymes generally function best at a veritable pH. The optimum pH changes depending on the enzyme in question. The enzyme experimented on in this experiment was the enzyme catalase, which works best at a pH of 7.0 (http://www.worthington-biochem.com/IntroBiochem/effectspH.html). If the pH of the substrate is changed even slightly the enzyme may father d enaturation (IB biology notes). Denatuation is the changing of the structure of an enzyme so that it stack no longer carry out its function, this is usually unchanging (IB biology notes). Variables:Dependent variable:Rate of catalase reaction when dropped into a sporting vial containing 20ml of hydrogen peroxide at common chord different pH levels. Independent variable:The pH of a 20ml clear vial of hydrogen peroxide. Controlled variable:Temperature exclusively tests were interpreted in the same room, at room temperature.

Time in all tests were interpreted on the same day, in the same 90 minute lab session. Light intensity All tests were taken under ! the same light intensity. Concentration of solutions All troika 20ml vials of hydrogen peroxide came from the same batch. Source of biological honest-to-god used All materials were taken from the same batch. Total spate of reaction mixtureAll liquids were poured from the same 10ml graduated cylinder. Materials:Notebook or seagull paperPen or pencil3 clear vials2 20ml hospital plastic cup100% Catalase solution, If you want to go forth a full essay, order it on our website: OrderCustomPaper.com

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